This cell line is a sub-strain of the mouse myoblast cell line established by Yaffe D. and Saxel O. It differentiates rapidly, forming contractile myotubes and producing specific muscle proteins. Under the influence of bone morphogenetic protein (BMP-2), this cell can differentiate from myoblasts into osteoblasts. Testing confirmed that the cells are negative for mousepox virus.

Cell Name: Mouse myoblast cell line

Cell Synonyms: C2C12; C2c12; C2-C12; C12

Catalogue No.: TCM-C720

Species: Mouse
Tissue Origin: Muscle
Disease Characteristics: /
Morphology: Myoblast-like
Growth Characteristics: Adherent growth
Culture System: DMEM-H + 10% FBS + 1% Glutamax + 1% Sodium Pyruvate + 1% P/S
Matching Medium Code: TCM-G720
Passage Ratio: 1:3–1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma