C-127 is an untransformed clonal strain derived from RIII mouse mammary carcinoma. The supernatant of these cells does not exhibit reverse transcriptase activity. This cell line is commonly used for the presentation of sarcoma virus-induced aggregation and for in vitro quantitative assays of bovine papilloma virus. It has recently been frequently employed as a suitable recipient for bovine papilloma virus DNA plasmid transformation.

Cell Name: Mouse mammary epithelial cell line

Cell Synonyms: C127; C-127

Catalogue No.: TCM-C719
Species: Mouse
Tissue Origin: Mammary Gland
Disease Characteristic: Breast Cancer

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G719

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection