Originating from microglial cells of C57BL/6 mice, these cells express the nuclear v-myc and chromosomal v-raf oncogenes, and surface-express the env gp70 antigen. They exhibit morphological, phenotypic, and functional characteristics of phagocytic cells.

Cell Name: Mouse microglial cell line

Cell Synonyms: BV2; BV-2

Catalogue No.: TCM-C718

Species: Mouse
Tissue Source: Brain
Disease Characteristics: /
Morphology: Polymorphic
Growth Characteristics: Semi-adherent growth
Culture System: DMEM-H + 10% FBS + 1% P/S
Matching Culture Medium Code: TCM-G718
Passage Ratio: 1:3–1:6; change medium every 2–3 days
Doubling Time: 48–72 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma