This cell line is derived from a pancreatic tumor (insulinoma) grown in a transgenic mouse. The mouse carries a pseudogene construct of the SV40 early gene regulated by the promoter of the rat insulin II gene. The cells are rich in insulin and contain small amounts of glucagon and somatostatin. They secrete insulin in response to glucose.

Cell Name: Mouse insulinoma cell line

Cell Synonyms: β-TC-6; beta-TC-6; beta-TC6; beta TC6; BetaTC6; betaTC6;βeta-TC-6

Catalogue No.: TCM-C716

Species: Mouse
Tissue Origin: Pancreas
Disease Characteristics: /
Morphology: Epithelial-like
Growth Characteristics: Adherent growth
Culture System: DMEM-H + 15% premium heat-inactivated fetal bovine serum + 1% P/S
Matching Culture Medium Code: TCM-G716
Passage Ratio: 1:3–1:6, change medium every 2–3 days
Doubling Time: 72–96 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma