The BALB/3T3 clone A31 cell line is one of several cell lines established in 1968 by S. A. Aaronson and G. T. Todaro from lysed 14–17 day old BALB/c mouse embryos. The BALB/3T3 clone A31 cells are particularly sensitive to contact inhibition, requiring high dilution for growth and exhibiting a low saturation density. In addition, these cells are highly susceptible to the oncogenic DNA virus SV40 and the mouse sarcoma virus.

Cell Name: Mouse embryo fibroblast cell line

Cell Synonyms: BALB/3T3 CLONE A31; BALB/c 3T3 clone A31; Balb/c3T3; Balb/c 3T3; BALB/3T3; Balb/3T3-4-Cl31; 3T3 clone A31; BALB/3T3 cl. A31; BALB 3T3 clone A31; BALB/3T3 (clone A31); B/C3T3; 3T3-A31; 3T3(A31)

Catalogue No.: TCM-C714

Species: Mouse

Tissue Origin: Embryo

Disease Characteristics: —

Morphology: Fibroblast-like

Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G714

Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection