B16-F10 is a subline of B16-F0

Cell Name: Mouse melanoma cell line

Cell Synonyms: B16-F10; B16/F10; B16 F10; B16F10; B16 melanoma F10

Catalogue No.: TCM-C713

Species: Mouse

Tissue Origin: Skin

Disease Feature: Melanoma

Morphology: Epithelial-like with spindle-shaped cells

Growth Characteristics: Adherent growth

Culture System: DMEM-H + 10% FBS + 1% P/S

Matching Culture Medium Catalog Number: TCM-G713

Passage Ratio: 1:3–1:6, change medium every 2–3 days

Doubling Time: 48–72 hours

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma