Product Name：Luciferase Reporter B16 Cell Line

HyCyte cell line：HyCyte B16-Luc

Catalog No.：TCM-C712L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：B16

Species：Mouse

Morphology：Epithelioid mixed with spindle cells

Growth properties：Adherent

Culture Medium：Dulbecco's Modified Eagle Medium ,High Glucose (DMEM) supplemented with 10 % FBS,200.0 ug/mLHygro , 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCM-G712L）

Passage：1:3-1:6

Doubling time：48-72 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).