The B16 cell line is a strain of mouse melanoma cells.

Cell Name: Mouse melanoma cell line

Cell Synonyms: B16; B-16; B16 melanoma; B16 subline B78; B78

Catalogue No.: TCM-C712
Species: C57BL/6 Mouse
Tissue Origin: —
Disease Characteristics: Melanoma
Morphology: Fibroblast-like

Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G712

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection