The AtT-20 cells do not adhere to the substrate during growth but instead form cell clusters and grow in suspension. Studies have shown that AtT-20 cells are resistant to poliovirus.

Cell Name: Mouse pituitary tumor cell line

Cell Synonyms: ATT-20; AtT20; AtT 20; AtT-20

Catalogue No.: TCM-C711
Species: Mouse
Tissue Origin: Pituitary
Disease Characteristics: Pituitary tumor
Morphology: Round
Growth Properties: Semi-adherent

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G711

Passage Ratio: 5×10⁵-1×10⁶ cells/mL, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection