The AML12 (α Mouse Liver 12) cell line was established from hepatocytes derived from transgenic mice (CD1 strain, line MT42) expressing human TGFα. Electron microscopy observations revealed that these cells exhibit typical hepatocyte characteristics, such as peroxisomes and bile canaliculus-like structures. AML12 cells retain the ability to express high levels of mRNA for serum proteins (albumin, α1-antitrypsin, and transferrin) and gap junction proteins (connexins 26 and 32), and they contain only lactate dehydrogenase isoenzyme 5. The cells express high levels of human TGFα and lower levels of mouse TGFα. The expression of liver-specific proteins decreases over time in culture but can be reactivated by culturing the cells in serum-free medium.

Cell Name: Mouse hepatocyte cell line

Cell Synonyms: AML12; AML-12; AML 12; Alpha Mouse Liver 12

Catalogue No.:TCM-C709
Species: Mouse
Tissue Origin: Liver

Disease Characteristics: —

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: D/F12 + 10% FBS + 1% ITS + 40 ng/mL Dexamethasone + 1% P/S
Matching Medium Catalog Number: TCM-G709

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection