Product Name：Luciferase Reporter AE-2 Cell Line

HyCyte cell line：HyCyte AE-2-Luc

Catalog No.：TCM-C708L

Construction method：Lentivirus

Selection Marker：Puromycin

Parental：AE-2

Species：Mouse

Morphology：Lymphoblast-like

Growth properties：Adherent

Culture Medium：Dulbecco's Modified Eagle Medium ,High Glucose (DMEM)    supplemented with 10 % FBS,4.0 ug/mL Puromycin, 100 U/ml Penicilin and 100 µg/ml Streptomycin (HyCyte,TCM-G708L）

Passage：3×10^5-5×10^5cells/mL

Doubling time：24-48 h

Subculture：Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Cells should be passaged every 2-3 days. Split at 80-85% confluency, approximately 1:3-1:6.

Freezing media：DMEM+ 30 % FBS + 10 % DMSO;or One Step Freezing Medium (HyCyte, GUCP-R201).