Immunize experimental animals with purified human erythrocyte acetylcholinesterase, and fuse the spleen cells with SP2/O-Ag14 myeloma cells. The resulting antibodies bind to acetylcholinesterase from both humans and monkeys, but the reaction site of the AE-2 cell is different from the antigenic determinant of AE-1. Testing confirmed that the AE-2 cell line is negative for short-limbed deformity (mousepox) virus.

Cell Name: Mouse hybridoma cells

Cell Synonyms: AE-2; AE2

Catalogue No.: TCM-C708

Species: Mouse

Tissue Origin: B lymphocyte

Disease Characteristic: Lymphoma

Morphology: Lymphoblast-like

Growth Properties: Suspension

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G708

Passage Ratio: 3×10⁵-5×10⁵ cells/mL, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection