The 15P-1 cell line was established from the testicular cells of transgenic mice, in which the large T protein of polyomavirus (PyLT) is expressed in spermatogenic cells.

Cell Name: Mouse testicular epithelial cells

Cell Synonyms: 15P-1

Catalogue No.: TCM-C700

Species: Mouse

Tissue Origin: Testis

Disease Characteristics: —

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G700

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection