266-6 is a cell line with an epithelial morphology, isolated from the pancreas of mice bearing pancreatic acinar cell tumors. The tumor was induced using an elastase I/SV-40 T antigen fusion gene. The cells retain a partially differentiated phenotype and express detectable levels of mRNA for various digestive enzymes. These cells respond to carbachol and cholecystokinin but do not respond to substance P, secretin, or vasoactive intestinal peptide (VIP). They carry an elastase I/neomycin transgene.

Cell Name: Mouse pancreatic acinar cell line

Cell Synonyms: 266-6; 2666

Catalogue No.: TCM-C586
Species: Mouse
Tissue Origin: Pancreas
Disease Characteristics: Acinar carcinoma
Morphology: Epithelial-like
Growth Properties: Adherent growth
Culture Medium: DMEM-H + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCM-G586
Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days

Doubling Time: —

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection