The SVG p12 cell line is derived from human fetal glial cells isolated from the brains of embryos aged 8 to 12 weeks, and was generated by transfection with DNA from an SV40 mutant. These cells express the SV40 T antigen. SVG p12 cells are susceptible to JC virus infection and can be used for the detection and cultivation of other human neurotropic viruses.

Cell Name: Human Brain Astrocytes

Cell Synonyms: SVG p12

Catalogue No.: TCH-C631
Species Origin: Human
Tissue Origin: Brain, astrocytoma; SV40-transfected
Disease Characteristics: Astrocytes
Morphology: Fibroblast-like

Growth Properties: Adherent growth

Culture Medium: : MEM + 10% FBS (fetal bovine serum) + 1% NEAA + 1% P/S

Passage Ratio: 1:2 to 1:4; change medium every 2–3 days
Doubling Time: 24–36 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended Cryopreservation Medium: HyCyte® One-Step Freezing Medium (ready-to-use, serum-free, no programmed cooling required), Product Code: GUCP-R201
Quality Testing: Negative for bacteria, fungi, and mycoplasma