$ 800.00
Immortalized human renal proximal tubule epithelial cell line(HK-2 [Human kidney])
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Cat.No.
TCH-C400
Size
1x10^6
Price
$800.00

The HK-2 cell line is derived from normal human kidney proximal tubular epithelial cells and was immortalized by the introduction of HPV-16 E6/E7 genes. A recombinant retroviral vector, pLXSN 16 E6/E7, containing the HPV-16 E6/E7 genes, was transfected into the amphotropic packaging cell line Psi-2. The virus produced by Psi-2 cells was then used to infect the ecotropic packaging cell line PA317. Finally, the viral particles generated by PA317 cells were introduced into normal human renal cortical proximal tubular epithelial cells. Although the pLXSN 16 E6/E7 vector carries a neomycin resistance gene, G418 selection was not used to isolate transduced clones. Southern blot and FISH analyses confirmed that the HK-2 cell line is derived from a single clone. PCR analysis verified the presence of the E6/E7 genes in the genome of HK-2 cells.


Cell Name: Immortalized human renal proximal tubule epithelial cell line (STR-authenticated)
Cell Synonyms: HK-2; Hk-2; HK2; Human Kidney-2

Catalogue No.: TCH-C400
Species: Human
Tissue Origin: Kidney

Disease Characteristics: --

Morphology:Epithelial-like
Growth Properties: Adherent

Culture Medium: MEM + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G400

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 24-48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection


Common Issues and Solutions in HK-2 Cell Culture

1.Vacuolation

The issue of vacuolation should be assessed in conjunction with cell growth rate and morphology:

  • If the growth rate and morphology are normal, vacuoles may be part of regular cellular activity and will diminish after passaging.

  • If growth is slow and morphology is abnormal, vacuoles may indicate autophagy. Increasing the serum ratio (not exceeding 20%) or switching serum brands may improve cell condition.

2.Filopodia Formation

Cells extending elongated filaments can occur in two scenarios:

  • First: Caused by cell migration. Cells are not static on the culture dish and may migrate. During migration, part of the cell remains in place, stretching out a thin filament. In this case, only a small number of cells exhibit filaments, and overall growth remains normal—no special treatment is needed.

  • Second: Caused by poor cell nutrition, where most cells show filaments and growth is slow. Increasing the serum ratio (not exceeding 20%) or adding 5 ng/mL EGF can help.


3.Abnormal Morphology

Normal HK-2 cells grow in a cobblestone-like pattern. If cells appear sickle-shaped or irregular, it may be due to significant changes in the culture environment (e.g., transitioning from serum-containing to serum-free conditions). Reverting to the original culture conditions can gradually restore cell morphology. If cells become elongated, it may indicate poor nutrition.


4.Slow Growth

Under standard culture conditions (MEM + 10% FBS + 1% PS) and a 1:3 passaging ratio, HK-2 cells typically require about 3 days per passage cycle.

  • If cell growth is slow (unable to passage within a week), evaluate whether the medium, especially the serum, is suitable.

  • Optimizing the medium and serum, along with increasing cell seeding density, can improve growth.

  • Variations in serum quality may affect cell condition, so using high-quality fetal bovine serum is recommended.


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