In 1984, J. Park and colleagues established the SNU-1 cell line from a poorly differentiated gastric carcinoma in situ that was removed prior to cytotoxic therapy. The cells are negative for L-dopa decarboxylase (DDC), positive for VIP receptors, but lack gastric receptors. No evidence of amplification or rearrangement of the N-myc, L-myc, myb, or EGF receptor genes was observed. The expression levels of c-myc and c-erb-B-2 RNA in these cells were comparable to those in other cell lines. The following genes were not expressed: N-myc, L-myc, c-cis, IGF-2, and gastrin-releasing peptide.

Cell Name: Human gastric carcinoma cell line

Cell Abbreviation: SNU-1; SNU1; NCI-SNU-1

Catalogue No.: TCH-C819
Species: Human
Tissue Origin: Stomach
Disease Characteristic: Gastric Cancer
Morphology: Round
Growth Properties: Suspension
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G819
Passage Ratio: 5×10⁵ - 1×10⁶ cells/mL, with medium renewal every 2-3 days
Doubling Time: 24-48 h

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma detection