Established in 1985 from the pleural effusion of a 62-year-old male patient with pulmonary giant cell carcinoma; it has been described as having c-myc amplification and KRAS mutation, and is capable of forming tumors in nude mice.
Alias: COR-L23P (P = primary); the cells are sensitive to doxorubicin, and multidrug-resistant subclones are available (COR-L23R, ACC 883).
Cell morphology: Epithelioid, very large cells, often multinucleated, growing in monolayer adherent culture.

Cell Name: Human Large Cell Lung Carcinoma Cells

Cell Synonyms: COR-L23

Catalogue No.: TCH-C638
Species : Human

Tissue Origin: Lung
Disease Characteristic: Lung cancer
Morphology: Epithelial-like

Growth Properties: Adherent growth

Culture Medium: RPMI-1640 + 10% FBS (fetal bovine serum) + 1% P/S
Passage Ratio: 1:2–1:4; change medium every 2–3 days
Doubling Time: 48–56 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required): GUCP-R201

Quality Control: Negative for bacteria, fungi, and mycoplasma.