Hepatitis B virus (HBV) is a DNA virus that causes acute and chronic necroinflammatory liver diseases and hepatocellular carcinoma. HBV is characterized by specific hepatotropism, non-cytopathic and non-lytic replication, and a tendency to develop chronic infection. HepG2.2.15 cells are a hepatoblastoma cell line capable of expressing HBV antigens and secreting complete HBV particles. They are currently the most suitable cell line for studying HBV replication and the response of hepatocytes to interferon (IFN).

Cell Name: Human Hepatocellular Carcinoma Cells (HBV+)
Cell Synonyms: HEP-G2/2.2.15; Hep-G2/2215; HepG2/2215; HepG2-2.2.15; HepG2 2.2.15; HepG2.2.15; HepG2(2.2.15); 2.2.15Product Code: TCH-C634

Catalogue No.: TCH-C216

Species: Human
Tissue Origin: Liver
Disease Characteristics: —
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: DMEM-H + 10% FBS (Fetal Bovine Serum) + 380 μg/ml G-418 + 1% NEAA + 1% P/S
Passage Ratio:1:2–1:3, with medium change every 2–3 days
Doubling Time: 36–48 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommendation:
Use HyCyte® One-Step Freezing Solution (ready-to-use, serum-free, no programmed cooling required), Product ID: GUCP-R201

Quality Control: Negative for bacteria, fungi, and mycoplasma
Notes: For cells received and cultured at ambient temperature, a 1:2 split ratio is recommended for the first subculture.