This cell line was isolated from the lung adenocarcinoma tissue (poorly differentiated, Grade III) of a 60-year-old Caucasian female patient. It has been reported to form tumors in immunodeficient mice, with most karyotypes being hypotetraploid, and is used in lung cancer research.

Cell Name: Human Poorly Differentiated Lung Adenocarcinoma Cells

Cell Synonyms:SK-LU-1
Catalogue No.:TCH-C632
Species: Human
Tissue Origin: Lung
Disease Feature: Lung adenocarcinoma
Morphology: Epithelial-like
Growth Properties: Adherent growth
Culture Medium: MEM + 10% FBS (Fetal Bovine Serum) + 1% NEAA + 1% P/S
Passage Ratio: 1:2–1:3; change medium every 2–3 days
Doubling Time: 48–60 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Recommended Cryopreservation Medium: HyCyte® One-Step Cryopreservation Medium (Ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection.