Plat-E is a highly important ecotropic retroviral packaging cell line. Its full name is Platinum-E, and it is derived from the Human Embryonic Kidney (HEK) 293 cell line.

Function: It is primarily used for the rapid and transient production of high-titer retroviruses, and can also be employed for stable retrovirus production.

The viruses produced by the Plat-E packaging cell line express a single tropic envelope, which allows them to infect only mouse and rat cells.

Cell Name: Human Retroviral Packaging Cell

Cell Synonyms: Plat-E

Catalogue No.: TCH-C628

Species : Human

Tissue Origin: Embryonic Kidney
Disease Characteristics: —
Morphology: Epithelial-like

Growth Properties: Adherent growth

Culture Medium: : MEM + 10% FBS (Fetal Bovine Serum) + 1% P/S (Penicillin-Streptomycin)

Passage Ratio: 1:2–1:4; change medium every 2–3 days
Doubling Time: 24–36 hours
Digestion Time: 1–2 minutes
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO; stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma detection