Established in 1996 from the ascites of a 29-year-old female with diffuse large B-cell lymphoma. The patient had been diagnosed with advanced Hodgkin's lymphoma 16 years prior and experienced multiple relapses despite undergoing various chemotherapy and radiotherapy regimens before achieving complete remission. Literature describes this cell line as overexpressing BCL2, BCL6, and p53, classifying it as belonging to the ABC-like subtype of lymphoma (activated B-cell type). Exome and RNA sequencing data are available (see reference 18187 and exome/RNA sequence data).

Additionally, please note: The U-2932 cell line contains two distinct subpopulations, which can be distinguished by surface staining for CD19, CD20, and CD38 (see reference 18133). It is recommended to continuously monitor these subpopulations to prevent accidental loss during passaging.

Cell Name: Human B-cell Lymphoma Cells (STR-authenticated)

Cell Synonyms: : U-2932

Catalogue No.: TCH-C625
Species : Human

Tissue Origin:   Lymphoma
Disease Characteristics: —

Morphology: Lymphoblast-like; mostly single cells, with occasional small clusters

Growth Properties: Suspension growth

Culture Medium: RPMI-1640 + 10% FBS (fetal bovine serum) + 1% P/S
Passage Ratio: 3×10⁵ – 5×10⁵ cells/mL; medium change every 2–3 days
Doubling Time: 36–48 hours
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Cryopreservation Conditions: 60% basal medium + 30% FBS + 10% DMSO; stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma
Precautions: For first-time receipt at room temperature, it is recommended to passage the cells at a 1:2 ratio.