THLE-2 is an epithelial cell line isolated from the left lobe of a donor. The TTHLE-2 (ATCC: CRL-2706) and THLE-3 (ATCC: CRL-11233) cell lines were derived from normal primary hepatocytes by infection with the SV40 large T antigen. [RF84749] This virus was introduced into the amphitropic packaging cell line PA317 by incorporating the Bgl I-Hpa I fragment of the SV40 T antigen. [RF84750]

THLE-2 and THLE-3 cells exhibit phenotypic characteristics of normal adult liver epithelial cells. When injected into athymic nude mice, they do not form tumors, possess a near-diploid karyotype, and do not express alpha-fetoprotein (AFP). [RF84750] These cells metabolize benzo[a]pyrene, N-nitrosodimethylamine (NDMA), and aflatoxin B1 into their ultimate carcinogenic metabolites, which can bind to DNA, indicating a functional cytochrome P450 pathway. [RF84750] Other enzymes involved in chemical carcinogen metabolism, such as epoxide hydrolase, NADPH-cytochrome P450 reductase, superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase, are also retained in THLE cells.

Cell Name: Immortalized human liver epithelial cell line (STR-authenticated)
Cell Synonyms: THLE-2; THLE2; Transformed Human Liver Epithelial-2

Catalogue No.: TCH-C620
Species: Human
Tissue Origin: Liver, Left Lobe
Disease Characteristics: —
Cell Morphology: Epithelial
Growth Properties: Adherent
Culture Medium: THIE-2 Basal Medium + 10% FBS + 1% Cell Growth Factors + 1% P/S
Matching Medium Catalog Number.: TCH-G620
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 36-48 h

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma detection