MRC-5 is a diploid human cell line composed of fibroblasts, derived from the lung tissue of a 14-week-old aborted white male fetus. The cells exhibit a uniform spindle-shaped morphology.

Using advanced dual transduction technology involving the SV40 large T antigen gene and hTERT (human telomerase reverse transcriptase), Hycyte has successfully developed the MRC-5/TERT-SV40 cell strain, which carries dual resistance to Puromycin (Puro) and Hygromycin (Hygro). This cell line not only retains the classic characteristics of the original MRC-5 cells but also demonstrates excellent passaging capability.

Cell Name: Immortalized human fetal lung fibroblast cell line

Cell Synonyms: MRC-5/TERT-SV40
Catalogue No.: TCH-C619
Species: Human
Tissue Origin: Lung
Disease Characteristics: /
Morphology: Fibroblast-like
Growth Properties: Adherent
Culture Medium: DMEM-H + 10% FBS + 1% P/S
Matching Medium Catalog Number : TCH-G619
Passage Ratio: 1:2 (based on the bottom area of the culture flask), with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO; store in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (Ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201

Quality Control: Negative for bacteria, fungi, and mycoplasma

Culture Notes:

1. Passaging Capability of MRC-5/TERT-SV40

   ① Cell condition is sensitive to medium changes. Please use the complete medium provided by Starfish (Product Code: TCH-G619). Using other media may affect cell morphology and passaging ability.

   ② When using the recommended complete medium and splitting at a ratio of 1:2, we guarantee at least 20 additional passages after receipt.

2. Trypsinization of MRC-5/TERT-SV40

   ① This cell line is sensitive to trypsin. Use Trypsin-EDTA (0.25%) with phenol red (NO. GUTP-R002) and incubate at 37°C for 1–2 minutes. Digestion time may vary slightly depending on cell density and brand of trypsin. Digest until approximately 80% of the cells become rounded and begin to detach. Over-digestion (where all cells detach) may occur if left too long, which can negatively impact cell health.

   ② A small number of floating cells may appear on the second day after passaging. This is normal and can be removed by changing the medium without affecting cell culture.