Established from the peripheral blood of a 20-year-old male with acute myeloid leukemia (AML, FAB M5a), who had relapsed in 1995 following an initial diagnosis of myelodysplastic syndrome (MDS, refractory anemia with excess blasts, RAEB). This cell line carries an internal tandem duplication (ITD) in the FLT3 gene; however, FLT3 protein is not expressed. It also harbors a CBL deltaExon8 mutant variant. MOLM-14 (ACC 777) is its sister cell line.

Cell Name: Human acute myeloid leukemia cell line (STR authenticated)
Cell Synonyms: MOLM-13; MOLM13; Molm13; Molm 13

Catalogue No.: TCH-C618
Species: Human
Tissue Origin: Peripheral Blood
Disease Characteristics: /
Morphology: Lymphoblast-like
Growth Properties: Suspension
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G618
Passage Ratio: 3×10⁵ - 5×10⁵ cells/mL, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Quality Control: Negative for bacteria, fungi, and mycoplasma
Precautions: For first-time receipt at room temperature, it is recommended to subculture at a 1:2 ratio.