This cell line is derived from human embryonic kidney cells 293F (where "F" stands for Fast-growth) transformed with SV40 large T antigen. It is a rapidly growing, highly transfectable clone isolate. It serves as an excellent host for producing high-titer lentiviruses and can stably express SV40 large T antigen from the pCMVSPORT6TAg.neo plasmid, with expression levels controlled by the human cytomegalovirus (CMV) promoter at relatively high levels.

Cell Name: Human embryonic kidney cell line, derivative of HEK 293 for virus production (STR-verified)
Cell Synonyms: 293FT; HEK-293FT; HEK 293FT; HEK-293-FT; HEK293FT; 293-FT; 293FT; FT-293

Catalogue No.: TCH-C617
Species: Human
Tissue Origin: Embryonic kidney
Disease Characteristics: /
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: DMEM-H + 10% FBS + 1% Glutamax + 1% NEAA + 1% P/S
Matching Medium Catalog Number: TCH-G617
Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 20-30 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂; Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Quality Control: Negative for bacteria, fungi, and mycoplasma

293FT Cell Culture Precautions

1. 293FT cells have weak adhesion and are prone to detachment. Handle with care. All supporting reagents must be pre-warmed before use. It is recommended to use culture flasks/dishes pre-coated with 0.1% gelatin for revival or passage. When changing the medium, avoid shaking the flask/dish forcefully. Add the medium slowly along the wall of the flask/dish to prevent direct pipetting onto the cells.

2. The recommended digestion time for 293FT cells is 0.5–1 minute. Avoid over-digestion.

3. 293FT cells grow very rapidly, typically with a doubling time of less than 24 hours. It is recommended to passage the cells when confluency reaches 70–90% to avoid over-confluence, which may lead to cell clumping.