Cell Name: EBV-transformed human lymphoblastoid cell lines (LCLs)

Cell Synonyms: NCI-BL1437; BL1437

Catalogue No.: TCH-C614
Species: Human
Tissue Oringin: Peripheral Blood
Disease Characteristics: —
Morphology: Lymphoblastoid
Growth Properties: Suspension
Culture Medium: D/F12 + 0.05 mg/mL Insulin + 0.01 mg/mL Transferrin + 30 nM Sodium Selenite + 10 nM Hydrocortisone + 10 nM Beta-Estradiol + 2 mM L-Glutamine + 5% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G614

Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection