The parental cell line is PC-3, derived from a 62-year-old Caucasian male with stage IV prostate cancer. This cell line (PC-3M) originated from a metastatic site: bone, and was isolated from tumors induced by PC-3 in mice. It shares a similar karyotype with PC-3 but displays a narrower distribution of chromosomal frequencies. Approximately 73% of PC-3/M cells contain 60 or 61 chromosomes.

Cell Name: Highly metastatic variant of PC-3

Cell Synonyms: PC-3M; PC3-M; PC-3/M; PC3M; Pc3M

Catalogue No.: TCH-C613

Species: Human

Tissue Origin: Human prostate cancer

Disease Characteristics: Prostate Cancer

Morphology: Epithelial, Polygonal

Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS + 1% P/S

Matching Medium Catalog Number: TCH-G613

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: —

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection