This cell line was established from the lung tissue of a 55-year-old Caucasian male. The cells are aneuploid, capable of forming colonies in soft agar, and retain the morphological and ultrastructural features of small cell carcinoma as well as APUD (amine precursor uptake and decarboxylation) cell characteristics. The cells grow in aggregates, which makes accurate cell counting difficult. They exhibit positive staining for cytokeratin. The N-myc gene is amplified, with both mRNA and protein expression present. In contrast, C-myc mRNA expression is low, and there is minimal or no C-myc protein expression.

The cells express mRNAs for c-myb, v-fes, v-fms, c-raf 1, Ha-ras, Ki-ras, and N-ras. They readily form tumors in nude mice that exhibit the typical histological features of small cell carcinoma. The cells also form colonies in soft agar.

Cell Name: Human small cell lung carcinoma cell line

Cell Synonyms: NCI-H69;NCI-H-69; NCI H69; H69; H-69; NCIH69; NCI-HUT-69; H69/P; NCI-H69C; H69C; H69c
Catalogue No.: TCH-C609
Species: Human
Tissue Origin: Lung
Disease Characteristics: —
Morphology: Multicellular aggregates
Growth Properties: Suspension
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number TCH-G609
Passage Ratio: 5×10⁵ -1×10⁶ cells/mL, with medium renewal every 2-3 days; passage 2-3 times per week
Doubling Time: 56 h

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection