This cell line was isolated in 1983 by Gazdar AF and Oie H from a chest wall tumor of a 73-year-old Caucasian male patient with adenosquamous carcinoma of the lung; the specimen was obtained prior to treatment. The cells express p53 at levels comparable to those found in normal lung tissue, with no major structural DNA abnormalities. They are positive for keratin and vimentin, but negative for neurofilament triplet proteins. The cells express various markers of squamous cell differentiation, such as glutamine transferase activity, production of involucrin, and formation of cross-linked envelopes.

Cell Name: Human adenosquamous lung carcinoma cell line

Cell Synonyms: NCI-H596
Catalogue No.: TCH-C608
Species: Human
Tissue Origin: Chest Wall Tumor
Disease Characteristics: Adenosquamous Carcinoma of the Lung
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G608
Passage Ratio: 1:4-1:8, 2-3 times per week
Doubling Time: 41.1 h

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection