SU-DHL-10 is a B lymphocyte cell line isolated in 1978 from the lymph node of a 25-year-old Caucasian male with large cell lymphoma. It is used in immunological research.

Cell Name: Human diffuse large B-cell lymphoma cell line

Cell Synonyms: SU-DHL-10; Su-DHL-10; SUDHL10; Sudhl10; SUDHL-10; SuDHL 10; Stanford University-Diffuse Histiocytic Lymphoma-10; DHL10

Catalogue No.: TCH-C605
Species: Human
Tissue Origin: Lymph Node
Disease Characteristic: Lymphoma
Morphology: Multicellular aggregates
Growth Properties: Suspension
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G605
Passage Ratio: 3 ×10⁵-5 ×10⁵ cells/mL, with medium renewal every 2-3 days

Doubling Time: 15-30 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection