Established from a well-differentiated esophageal squamous cell carcinoma with moderate adventitial invasion, resected from the cervical esophagus of a 67-year-old female after cisplatin and radiotherapy. Described as carrying a p53 mutation and amplifications of MYC, HST1, and CYCLIN D1, and capable of inducing well-differentiated tumors in nude mice.

Cell Name: Human esophageal squamous cell carcinoma cell line

Cell Synonyms: KYSE-510; KYSE 510; Kyse-510; KYSE510; Kyse510

Catalogue No.: TCH-C604
Species: Human
Tissue Origin: Esophageal squamous cell carcin

Disease Characteristics: Esophageal squamous cell carcinoma

Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G604

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: 24-30 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection