Cell Name: Human cholangiocarcinoma cell line

Cell Synonyms: HuCCT1; HuCCT-1; HUCCT-1; HUCC-T1; HUCCT1

Catalogue No.: TCH-C601

Species: Human

Tissue Origin: Bile duct, ascites metastasis

Disease Characteristics: Cholangiocarcinoma

Morphology: Epithelial-like

Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS + 1% P/S

Matching Medium Catalog Number: TCH-G601

Passage Ratio: 1:2, with medium renewal every 2-3 days
Doubling Time: 55 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection