The Hs 578T cell line originates from breast cancer. It shares the same patient origin as the normal fibroblast-like cell line Hs 578Bst. Initially, the Hs 578T cells exhibited a polygonal morphology; however, during subculturing, star-shaped cell morphology was selected and cloned. Under electron microscopy, aggregates of casein granules, desmosomes, tight junctions, lipid bodies, and smooth endoplasmic reticulum vesicles were observed. Like Hs 578Bst, no estrogen receptors or endogenous viruses were detected.

Cell Name: Human breast ductal carcinoma cell line

Cell Synonyms: Hs578T;HS 578T; Hs-578T; HS-578T; Hs_578t; Hs-578-T; HS-578-T; Hs 578.T; HS578T; Hs578T; Hs578t; HS0578T; 578T; HS578; Hs578; Homo sapiens No. 578, tumor cells
Catalogue No.: TCH-C600
Species: Human
Tissue Origin: Breast Cancer
Disease Characteristics: —
Morphology: Epithelial-like
Growth Properties: Adherent
Culture Medium: DMEM-H + 10% FBS + 0.01 mg/ml Insulin + 1% P/S
Matching Medium Catalog Number: TCH-G600

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 36-54 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection