The HNE-1 cell line was derived from a poorly differentiated squamous cell carcinoma of the nasopharynx. Desmosomes were observed under electron microscopy. The cells have been passaged over 100 times. EBV virus testing was negative.

Problematic cell line: Likely contaminated. The STR profile is highly similar to that of the HeLa cell line and nearly identical to that of HNE-2 (Cellosaurus = CVCL_FA07).

Cell name: Human nasopharyngeal carcinoma cell line (STR-verified, HeLa-contaminated cell line)

Cell Synonyms: HNE1; HNE-1

Catalogue No.: TCH-C599

Species: Human

Tissue Origin: Nasopharyngeal tissue

Disease Characteristics: Nasopharyngeal carcinoma

Morphology: Epithelial-like

Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS + 1% P/S

Matching Medium Catalog Number: TCM-G599
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: —

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection