The ES-2 cell line was established from a surgical tumor specimen of a 45-year-old Black female. The tumor was a poorly differentiated ovarian clear cell carcinoma. The cells were initially grown in soft agar and formed tumors in nude mice. The cells exhibit low to moderate resistance to various chemotherapeutic agents, including doxorubicin, cisplatin, carmustine, etoposide, and cyanomorpholinodoxorubicin (MRA-CN). ES-2 cells express low levels of P-glycoprotein.

Cell Name: Human ovarian clear cell carcinoma cell line

Cell Synonyms: ES-2; ES2
Catalogue No.: TCH-C596
Species: Human
Tissue Origin: Ovary
Disease Characteristics: Clear cell carcinoma
Morphology: Fibroblast-like
Growth Properties: Adherent
Culture Medium: McCoy’s 5A + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G596

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 28-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection