This cell line is a subclone of the human colorectal adenocarcinoma cell line COLO 320DM, also derived from round-shaped cells isolated from the colon of a 55-year-old Caucasian female patient with colorectal adenocarcinoma. The cell is characterized by prominent intrachromosomal amplification (homogeneously staining regions, HSR) and relatively low levels of extrachromosomal amplification (double minutes, DM), hence its name. It has been reported that this cell line can form tumors in nude mice, shows negative expression for CEA and CSAp, positive expression for colon antigen 3, and weakly positive for keratin and vimentin.

Cell Name: Human colorectal adenocarcinoma cell line (STR-authenticated)
Cell Synonyms: COLO 320HSR; COLO-320HSR; COLO #320HSR COLO320HSR; COLO 320 HSR; COLO-320-HSR; COLO 320 (HSR); COLO-HSR; Colorado 320 Homogeneously Staining Regions

Catalogue No.: TCH-C594
Species: Human
Tissue Origin: Colon
Disease Characteristics: —
Morphology: Round, clustered growth
Growth Characteristics: Loosely adherent or suspension
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G594
Passage Ratio: 1:2-1:3, with medium renewal every 2-3 days
Doubling Time: 48-72 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen
Quality Control: Negative for bacteria, fungi, and mycoplasma