Caki-2 cells were derived from the primary renal adenocarcinoma tissue of a 69-year-old Caucasian male.

Cell Name: Human renal clear cell carcinoma cell line

Cell Synonyms: Caki-2; CAKI-2; CaKi-2; caki-2; CAKI 2; Caki 2; Caki2; CAKI2

Catalogue No.: TCH-C592

Species: Human

Tissue Origin: Kidney

Disease Characteristics: Renal Clear Cell Carcinoma

Morphology: Epithelial-like

Growth Properties:Adherent

Culture Medium: McCoy's 5A + 10% FBS + 1% P/S

Matching Medium Catalog Number: TCH-G592

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 30-40 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection