Cell Name: Human T cell lymphoma cell line

Cell Synonyms: C8166-CD4; C8166
Catalogue No.: TCH-C591
Species: Human
Tissue Origin: T Lymphocytes
Disease Characteristics: Leukemia
Morphology: Lymphoblast-like
Growth Properties: Suspension
Culture Medium: RPMI-1640 + 10% FBS + 1% L-Glutamine + 1% P/S
Matching Medium Catalog Number: TCH-G591

Passage Ratio: 1:2, with medium renewal every 2-3 days
Doubling Time: —

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection