This cell line was established in 1958 by E.Y. Lasfargues and L. Ozzello, derived from the breast cancer tissue of a 74-year-old Caucasian female. The cells express WNT3 and WNT78. TNF-alpha inhibits the growth of these cells. The cells are estrogen receptor-negative but express estrogen mRNA with a 5' exon deletion.

Cell Name: Human breast ductal carcinoma cell line

Cell Synonyms: BT-20; BT 20; BT20

Catalogue No.: TCH-C590

Species: Human

Tissue Origin: Breast

Disease Characteristics: Breast Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCM-G590
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 70 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection