The AN3CA cell line was established in 1964. Derived from lymph node metastatic tissue of an endometrial cancer patient, it exhibits fundamental characteristics of cancer cells, capable of long-term in vitro subculture and demonstrating tumorigenicity in mice. However, its biological properties and ultrastructure have not been extensively studied, with only negative melanotropin synthesis being identified in this cell line. These cells are commonly used for research on the biology of human endometrial cancer cells and their related characteristics.

Cell Name: Human endometrial adenocarcinoma cell line

Cell Synonyms: AN3CA; AN3 CA; AN3 Ca; AN-3; AN3, AN3-CA
Catalogue No.: TCH-C589
Species: Human
Tissue Origin: Uterus
Disease Characteristics: Endometrial Cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 15% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G589
Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 55 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection