The NCI-H2122 [H2122] cell line is a lymphoblastoid cell isolated in 1989 from the pleural effusion metastasis of a 46-year-old female smoker.

Cell Name: Human non-small cell lung adenocarcinoma cell line

Cell Synonyms: NCI-H2122; H2122; H-2122; NCIH2122

Catalogue No.: TCH-C583
Species: Human
Tissue Origin: Lung
Disease Characteristics: Adenocarcinoma; Non-small cell lung cancer
Morphology: Lymphoblastoid
Growth Properties: Semi-adherent and semi-suspension
Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G583

Passage Ratio: 1:3-1:4, with medium renewal every 2-3 days
Doubling Time: 27 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection