The HS 683 cell line was derived from the left temporal lobe glioma tissue of a 76-year-old Caucasian male; HS 683 cells possess microvilli but lack desmosomes.

Cell Name: Human Glioma Cells
Cell Synonyms: Hs 683; HS 683; HS-683; Hs-683; Hs683; HS683; Hs 683.T; HS 683T; Hs683T

Catalogue No.: TCH-C582
Species: Human
Tissue Origin: Brain
Disease Characteristic: Glioma
Morphology: Fibroblast-like

Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G582
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 49-96 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection