Established from a 62-year-old Japanese male with bone marrow diffuse large B-cell lymphoma.

Cell Name: Human diffuse large B-cell lymphoma cell line (No STR data)
Cell Synonyms: TMD8; TMD-8; Tokyo Medical and Dental university 8

Catalogue No.: TCH-C581

Species: Human
Tissue Origin: Bone marrow
Disease Characteristic: Bone marrow diffuse large B-cell lymphoma
Morphology: Lymphoblast-like
Growth Properties: Suspension

Culture Medium: RPMI-1640 + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G581

Passage Ratio: 5×10⁵ -1×10⁶ cells/mL, with medium renewal every 2-3 days
Doubling Time: 36-48 h

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C
Freezing Conditions: 60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Quality Control: Negative for bacteria, fungi, and mycoplasma.