The NCI-H295R cell line is derived from the pluripotent adrenocortical cancer cell line NCI-H295, which was established by Gazdar A.F. et al. and isolated from an adrenal cortical tumor. The NCI-H295 cells were modified under altered culture conditions to yield the NCI-H295R cell line, with the population doubling time reduced from the original 5 days to 2 days. While the NCI-H295 cells grow in suspension, the NCI-H295R cells exhibit monolayer adherent growth. The NCI-H295R cell line retains the ability to produce androgens and responds to angiotensin II and potassium ions. Age (sex): 48 years.

Cell Name: Human adrenocortical carcinoma cell line

Cell Synonyms: NCI-H295R; NCI H295R; NCIH295R; H295R; H-295R; H295R-S1

Catalogue No.: TCH-C578
Species: Human
Tissue Origin: Organ, Adrenal Gland
Disease Characteristics: Adrenocortical Carcinoma

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM/F12＋10% FBS＋1% ITS＋1% P/S

Matching Medium Catalog Number: TCH-G578
Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: —

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection