The Hs-746T cell line was established in 1973. The size, shape, and nuclear morphology of Hs-746T cells can change during the culture process. Due to the malignancy of Hs-746T cells, their karyotype is often hypotriploid (46%).

Cell Name: Human gastric carcinoma cell line

Cell Synonyms: Hs 746.T; HS 746T; Hs 746T; HS 746T; Hs-746T; HS-746T; Hs746T; HS746T; Hs746-T; 746T

Catalogue No.: TCH-C577
Species: Human
Tissue Origin: Stomach; derived from metastatic site: left leg
Disease Characteristics: Gastric cancer

Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: DMEM-H + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G577

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: —

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

Freezing Conditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection