C3A is a clonal derivative of Hep-G2, characterized by strong growth inhibition, high albumin production, elevated alpha-fetoprotein (AFP) secretion, and the ability to grow in glucose-deprived culture medium.

Cell Name: Human hepatocellular carcinoma cell line, subclone of Hep G2(Hep-G2/C3A)

Cell Synonyms: C3A
Catalogue No.: TCH-C569
Species: Human
Tissue Origin: Liver
Disease Feature: Liver Cancer
Morphology: Fibroblast-like

Growth Properties: Adherent

Culture Medium: MEM + 10% FBS+ 1% P/S
Matching Medium Catalog Number: TCH-G569

Passage Ratio: 1:3-1:6, with medium renewal every 2-3 days
Doubling Time: —

Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection