A human T cell lymphoma cell line established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin's lymphoma in 1986; now classed as CD30+ anaplastic large cell lymphoma (ALCL); cells carry the NPM-ALK fusion gene.

Cell Name: Human anaplastic large cell lymphoma cell line

Cell Synonyms: Karpas-299; KARPAS-299; Karpas 299; KARPAS 299; Karpas299; KARPAS299; K299

Catalogue No.: TCH-C567

Species: Human

Tissue Origin: Peripheral Blood

Disease Characteristic: Lymphoma

Morphology: Lymphoblast-like

Growth Properties: Suspension

Culture Medium: RPMI-1640 + 10% FBS + 1% P/S

Matching Medium Catalog Number: TCH-G567

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 26-30 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection