A permanent hematopoietic cell line, designated NALM-1, was established from the peripheral blood of a patient with Ph1-positive chronic myelocytic leukemia in blastic crisis.

Cell Name: Human pre-B cell leukemia cell line

Cell Synonyms: Nalm-1
Catalogue No.: TCH-C561
Species: Human
Tissue Origin: Peripheral Blood
Disease Characteristic: Leukemia
Morphology: Lymphoblast-like
Growth Properties: Suspension
Culture Medium: RPMI-1640 + 20% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G561

Passage Ratio: 4.5×10⁵-6×10⁵ cells/mL, with medium renewal every 2-3 days
Doubling Time: 96-120 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection