Discovered in the pleural effusion of a 65-year-old male patient with lung adenocarcinoma, a type of non-small cell lung cancer

Cell Name: Human non-small cell lung squamous cell carcinoma cell line

Cell Synonyms: HCC78; HCC-78; HCC0078; Hamon Cancer Center 78

Catalogue No.: TCH-C558
Species: Human
Tissue Origin: Lung
Disease Characteristic: Non-small cell lung cancer
Morphology: Epithelial-like
Growth Properties: Adherent

Culture Medium: RPMI-1640 + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G558

Passage Ratio: 1:2-1:4, with medium renewal every 2-3 days
Doubling Time: 60-70 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection